Ceropegia, Brachystelma and Riocreuxia in Southern Africa: by R. Allen Dyer

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By R. Allen Dyer

This article is a pictorial presentation and incorporates a key to genera and species.

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The location of mimosine spot from the sample on the developed chromatographic paper must be carefully authenticated with standard mimosine. References 1. , and Sherman, G. D. (1951) A rapid colorimetric method for the determination of mimosine. Arch. Biochem. Biophys. 33, 195–200. 2. , Vargheese, C. , and Balasubramanian, N. (1993) Spectrophotometric determination of mimosine and 3-hydroxy-4-(1H)-pyridone—the toxic principles of Leucaena leucocephala. Anal. Biochem. 213, 57–62. ; Sesbania spp.

10. Transfer a 5-mL aliquot to another 100-mL volumetric flask and dilute to approximately 70 mL with distilled water. 11. 5M KSCN and bring the volume to 100 mL with distilled water, and read the color immediately (within 1 min) at 480 nm using a spectrophotometer. 12. Run a reagent blank with each set of samples. 1. 5 mL of the stock Fe(NO3)3 solution and make the volume up to 250 mL in a volumetric flask. 5-, 5-, 10-, 15- and 20-mL aliquots of this working standard into a series of 100-mL volumetric flasks and dilute them to approximately 70 mL with distilled water.

4. Transfer Cd with distilled water to a high-speed blender and blend for 2 to 3 s. 5. Retain 8 to 40 mesh particles and repeat blending to increase yield of particles. 6. 1 N HCl, stir occasionally with a glass rod, and leave it overnight in acid. 7. Stir once to degas and decant. Then wash with two 500-mL portions of distilled water. 8. Fill the modified Jones reductor in a chromatographic column (glass tube of approximately 300 mm in length and 10 mm in internal diameter) plugged with a glass wool and fixed with a stopcock.

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