Aging in Cell and Tissue Culture: Proceedings of a symposium by Emma Holečková, Vincent J. Cristofalo (auth.), Emma

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By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)

The annual assembly of the eu Tissue tradition ., Society was once held on the citadel of Zinkovy in Czechoslovakia from may perhaps 7-10,1969. incorporated as a part of this assembly used to be a symposium on "Aging in mobile and Tissue Culture." This quantity comprises the papers awarded at that symposium. using cellphone and tissue tradition concepts to review the mechanism of getting older isn't new. for instance, it has lengthy been recognized that age-associated adjustments which take place in plasma can inhibit telephone proliferation in vitro; additionally that the time lapse ahead of mobilephone migration from ex­ planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, cognizance has been interested in the incidence of senescence in vitro. those investi­ gations have incorporated experiences of adjustments in non­ dividing cellphone cultures, and to a a little bit higher volume, of age-related adjustments within the proliferative skill of cells in vitro. for instance, cells derived from human fetal lung keep many houses of standard cells together with a reliable general diploid karyotype and those cultures were proven to have a restricted life-span in vitro. In addi­ tion, cultures derived from human grownup lung express an identical general features and seem to have a shorter existence­ span than cells derived from fetal lung.

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Extra resources for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969

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The negative correlation between mean and standard deviation is obvious. 19 standard deviation Fig. 5. Correlation between mean log cell volume and standard deviation of Potorous tridactyl is embryonic heart cells during early passage • • cell populations from experiment 2~ passage 12-14 o cell populations from experiment 3~ passage 11-14 33 CELL VARIABILITY AND AGING When the correlation between mean and standard deviation is negative for young cells and positive for old cells, the product of the standard deviation and the mean ( m x s) might be a better indicator for cell aging than each parameter alone.

Coon, "An established cell line of fibrobroblasts from goose cells, " Carnegie Institution Year Book, 67: 421, 1969. 16. G. J. Todaro, S. R. Wolman, and H. Green, "Rapid transformation of human fibroblasts with low growth potential into established lines by SV40," J. Cell. , 62: 257, 1963. 17. G. J. Todaro and H. Green, "Serum albumin supplemented medium for long-term cultivation of mammalian fibroblast strains," Froc. Soc. Expti. BioI. , 116: 688, 1964. 18. K. H. Rothfels, E. B. Kupelwieser, and R.

Canc. , 32:1031, 1964. 22. D. Yaffe, "Retention of differentiation potentialities during prolonged cultivation of myogenic cells, " Froc. Nati. Acad. , 61: 477, 1968. 23. D. Yaffe and C. Revivi, to be published. 24. H. G. Coon, "Clonal culture of differentiated rat liver cells, " J. , 39: 29a, 1968. 25. H. G. Coon, personal communication. 26. L. Siminovitch, J. E. Till, and E. A. McCulloch, "Decline in colony-forming ability of marrow cells subj ected to serial transplantation into irradiated mice," J.

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