2011-2012 Basic and Clinical Science Course, Section 4: by Robert H. Rosa Jr., MD

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By Robert H. Rosa Jr., MD

This quantity is split into elements: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: scientific facets. half I makes use of a hierarchy that strikes from common to express to assist derive a differential analysis for a particular tissue. half II is a compilation of chosen scientific points of significance to the final ophthalmologist. Following half II are the yank Joint Committee on melanoma 2010 staging kinds for ocular and adnexal tumors. This revised textual content comprises a variety of new pathologic and medical pictures. significant revision 2011-2012.

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Extra resources for 2011-2012 Basic and Clinical Science Course, Section 4: Ophthalmic Pathology and Intraocular Tumors (Basic & Clinical Science Course)

Sample text

D, Step C is repeated multiple times to generate thousands of copies. (Courtesy of Theresa Kramer. ) CHAPTER 4: Special Proced ures. 41 relies on thermal cycles of repeated heating and cooling of the DNA sample for D A melting and enzymatic replica tion. Primers, which are short DNA fragments containing sequences complementary to the ta rget region, DNA polymerase, and nucleotides are the components required in order for selective and repeated amplification to occur. The selectivity of PCR is due to the use of primers that are complementar y to the DNA region targeted fo r ampli fication.

Histologically, noncaseating gra nulomatous "tubercles" (round to oval aggregates of epithelioid histiocytes) are present within the conjunctival stroma with a variable, but usually minimal, cuff of lymphocytes and plasma cells. Multinucleated giant cells may or may not be present within the granulomas. Central necrosis is not characteristic and, if present, should suggest infectious causes of granulomatous inflammation. The diagnosis of sarcoidosis is tenable only when supported by clinical findin gs and after other causes of granulomatous inflammation have been excluded by histochemical stains andlor by culture.

Tissue Processing The infiltration and embedding process removes most of the water from the tissue and re places the water with paraffin. OrganiC solvents used in this process will dissolve lipid and may dissolve some synthetic materials. Routine processing usually dissolves intraocular lenses made of polymethylmethacrylate (PMMA) , polypro pylene, and silicone, altho ugh the PMMA may fall out during sectioni ng. Silk, nylon, and other synthetiCsutures do not dissolve during routine processing.

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